Sashital lab publications (link to Google Scholar)

Murugan, K., Seetharam, A.S., Severin, A.J., & Sashital, D.G. (2019) Pervasive off-target and double-stranded DNA nicking by CRISPR-Cas12a. bioRxiv, 657791. Preprint link

Lee, H., Dhingra, Y., & Sashital, D.G.. (2019) The Cas4-Cas1-Cas2 complex mediates precise prespacer processing during CRISPR adaptation. eLife. e44248. link

Suresh, S.K., Murugan, K., & Sashital, D.G.. (2019) Enzymatic anti-CRISPRs improve the bacteriophage arsenal. NSMB. 26, 250-251. News & Views article. link($)

Xue, C. & Sashital, D.G.. (2019) Mechanisms of Type I-E and I-F CRISPR-Cas Systems in Enterobacteriaceae. EcoSal Plus. 8. Review article. link ($)

Phan, P., Schelling, M., Xue, C., & Sashital, D.G.. (2018) Fluorescence-based methods for measuring target interference by CRISPR–Cas systems. Methods in Enzymol. 616, 61-85. link($)

Mekler, V., Kuznedelov, K., Minakhin, L., Murugan, K., Sashital, D.G., & Severinov, K. (2018) CRISPR–Cas molecular beacons as tool for studies of assembly of CRISPR–Cas effector complexes and their interactions with DNA. Methods in Enzymol. 616, 337-363. link($)

Davis-Vogel, C., Van Allen, B., Van Hemert, J.L., Sethi, A., Nelson, M.E., & Sashital, D.G.. (2018) Identification and comparison of key RNA interference machinery from western corn rootworm, fall armyworm, and southern green stink bug. PLoS One. 13:e0203160. link

Davis-Vogel, C., Ortiz, A., Procyk, L., Robeson, J., Kassa, A., Wang, Y., Huang, E., Walker, C., Sethi, A., Nelson, M.E., & Sashital, D.G.. (2018) Knockdown of RNA interference pathway genes impacts the fitness of western corn rootworm. Sci Rep. 8, 7858. link

Sashital, D.G. (2018) Pathogen detection in the CRISPR-Cas era. Genome Med, 10, 32. Preview Article link

Lee, H., Zhou, Y., Taylor, D.W., & Sashital, D.G. (2018) Cas4-Dependent Prespacer Processing Ensures High-Fidelity Programming of CRISPR Arrays. Mol Cell. 70, 1-12. link($)

Xue, C., Zhu, Y., Zhang, X., Shin, Y.K., & Sashital, D.G. (2017) Real-time observation of target search by the CRISPR surveillance complex Cascade. Cell Rep. 21, 3717-3727. link

Murugan K., Babu K., Sundaresan R., Rajan R., & Sashital D.G. (2017) The Revolution Continues: Newly Discovered Systems Expand the CRISPR-Cas Toolkit. Mol Cell. 68, 15-25. Review Article link($)

Sashital, D.G. (2017) Prokaryotic Argonaute Uses an All-in-One Mechanism to Provide Host Defense. Mol Cell. 65, 957-958. Preview Article link($)

Xue, C., Whitis, N.R., & Sashital, D.G. (2016) Conformational Control of Cascade Interference and Priming Activities in CRISPR Immunity. Mol Cell. 64, 1-9. link($)

Wolt, J.D., Wang, K., Sashital, D.G., & Lawrence-Dill, C.J. (2016) Achieving Plant CRISPR Targeting that Limits Off-Target Effects. Plant Genome. 9. Review Article link

Xue, C., Seetharam, A.S., Musharova, O., Brouns, S.J.J., Severinov, K., Severin, A.J., & Sashital, D.G. (2015) CRISPR interference and priming varies with individual spacer sequences. Nuc Acids Res. 43, 10831-47. link


Pre-ISU publications

Sashital, D.G.*, Greeman, C.A.*, Lyumkis, D., Potter, C.S., Carragher, B., & Williamson, J.R. (2014) A combined quantitative mass spectrometry and electron microscopy analysis of ribosomal 30S subunit assembly in E. coli. eLife. 3:e04491. *Equal contribution link

Sashital, D.G., Wiedenheft, B., & Doudna, J.A. (2012) Mechanism of foreign DNA selection in a bacterial adaptive immune system. Mol Cell. 46, 606-15. link

Burke, J.E., Sashital, D.G., Zuo, X., Wang, Y., & Butcher, S.E. (2012) Structure of the yeast U2/U6 snRNA complex. RNA. 18, 673-83. link

Sashital, D.G., Jinek, M., & Doudna, J.A. (2011) An RNA-induced conformational change required for CRISPR RNA cleavage by the endoribonuclease Cse3. Nat Struct Mol Biol. 18, 680-7. link($)

Sashital, D.G. & Doudna, J.A. (2010) Structural insights into RNA interference. Curr Opin Struct Biol, 20, 90-7. Review Article link

Sashital, D.G. & Butcher, S.E. (2007) Is the spliceosome a ribozyme? In D.M.J. Lilley and F. Eckstein (Eds.), Ribozymes and RNA catalysis (pp. 253-266). Cambridge: The Royal Society of Chemistry. Book Chapter

Sashital, D.G., Venditti, V., Angers, C.G., Cornilescu, G., & Butcher, S.E. (2007) Structure and thermodynamics of a conserved U2 snRNA domain from yeast and human. RNA, 13, 328-38. link

Sashital, D.G. & Butcher, S.E. (2006) Flipping off the riboswitch: RNA structures that control gene expression. ACS Chem Biol, 1, 341-5. Preview Article link($)

Sashital, D.G., Cornilescu, G., McManus, C.J., Brow, D.A. & Butcher, S.E. (2004) U2-U6 RNA folding reveals a group II intron-like domain and a four-helix junction. Nat Struct Mol Biol, 12, 1237-42. link($)

Sigel, R.K., Sashital, D.G., Abramovitz, D.L., Palmer, A.G., Butcher, S.E., & Pyle, A.M. (2004) Solution structure of domain 5 of a group II intron ribozyme reveals a new RNA motif.  Nat Struct Mol Biol, 11, 187-92. link($)

Sashital, D.G., Allmann, A.M., Van Doren, S.R., & Butcher, S.E. (2003) Structural basis for a lethal mutation in U6 RNA. Biochemistry, 42, 1470-7. link